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KMID : 0617219930040010006
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Duksung Bulletin Phamaceutical Sciences
1993 Volume.4 No. 1 p.6 ~ p.11
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Inhibition of Benzo(a)pyrene-Induced Mutagenicity and Lipid Peroxidation by d-Limonene and Cineole
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Abstract
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It has been reported that d-limonene inhibits chemical-induced rat mammary cancer by the mechanism of increases in detoxification enzymes such as glutathione S-transferases and that cineole fails to exhibit significant suppressive effect on chemical-induced carcinogenesis. The present study was designed to compare the effects of d-limonene and cineole on the benzo(a)pyrene (BP)-induced mutagenicity, BP metabolism and lipid peroxidation. Modified Ames assay was employed to evaluate the inhibitory effect of d-limonene and cineole on the BP-induced mutagenicity. The number of revertant-bearing wells was decreased by 44∼77％ in the presence of both BP and d-limonene compared with that of BP alone whereas cineole decreased the number of revertant-bearing wells by 28∼45％ at the concentrations between 2㎛ and 2mM. d-Limonene suppressed BP metabolism by 16, 54 and 67％ at 1, 10 and 100 mM, respectively while cineole inhibited the metabolism by 16, 26 and 55％ at the same concentrations. The EC_50 values for d-limonene and cineole in inhibiting lipid peroxidation were 2.0 mM and 16 mM respectively, as assayed by thiobarbituric acid method. The present study showed that d-limonene and cineole have common antimutagenic effects although d-limonene appeared to be more effective than cineole in suppressing mutation and lipid peroxidation. The results suggest that the antimutagenic effects of d-limonene and cineole may be associated with alternation in enzyme activities and with inhibition of lipid peroxidation.
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KEYWORD
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